|
KMID : 0358420100530100905
|
|
Korean Journal of Obstetrics and Gynecology
2010 Volume.53 No. 10 p.905 ~ p.914
|
|
|
The effect of granulocyte-macrophage-stimulating factor on the gene expression in human trophoblast of early pregnancy
|
|
Lee Hun-Yul
Kim Sang-Soo
An Chang-Sook
Park Young-Joo
Jun Jin-Hyun
Song Haeng-Suk
Moon Hye-Sung
Park Eun-Joo
Hong Seo-Yu
Park Won-Il
|
|
|
Abstract
|
|
|
Objective: Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates mammalian embryonic development and implantation. However, its biological function after implantation is not elucidated. The aim of this study is to assess the changes of gene expression by GM-CSF in human trophoblast obtained in early pregnancy.
Methods: Human trophoblast obtained in early pregnancy was cultured with or without GM-CSF. The difference of gene expression was evaluated with microarray and selected genes were reevaluated with real-time reverse transcription-polymerase chain reaction (RT-PCR).
Results: Microarray analysis revealed that the expressions of 468 genes were increased while those of 40 genes were decreased by GM-CSF. These genes were evaluated according to the known biologic pathways. The regulation of actin cytoskeleton and focal adhesion pathways were mostly influenced by GM-CSF. Annexin A2, thymosin-like 3, vimentin, myogenin, ACK1, and tensin1 genes were selected for real-time RT-PCR. The increased expressions of of vimentin and ACK1, and decreased expressions of tensin1 were confirmed by real-time RT-PCR.
Conclusion: GM-CSF activates focal adhesion pathway in human trophoblast by increasing the expression of vimentin and ACK1, and decreasing the expression of tensin1.
|
|
|
KEYWORD
|
|
|
Granulocyte-macrophage colony-stimulating factor, Trophoblast, Focal adhesion, Invasion
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|